Highly directed differentiation of stem cells requires cell culture in a temporally defined series of variable media.
Click here for a brief animated tutorial explaining how CombiCult™ enables massive screens that rapidly identify the optimal media combinations to generate any specific cell type.
Module 1. Stem cells are seeded onto microscopic beads.
Module 2. Beads are shuffled through multiple differentiation media spiked with fluorescent tags that label the beads.
Module 3. Beads are screened to identify those bearing differentiated cells (hits).
Module 4. Hits are sorted by COPAS into the wells of a microtitre plate.
Module 5. Tags are analysed by FACS to deconvolute the cell culture history of hits.
Module 6. Ariadne™ bioinformatics software ranks the differentiation protocols.